Nucleosome Turnover Regulates Histone Methylation Patterns over the Genome.
Mol Cell
; 73(1): 61-72.e3, 2019 01 03.
Article
en En
| MEDLINE
| ID: mdl-30472189
ABSTRACT
Recent studies have indicated that nucleosome turnover is rapid, occurring several times per cell cycle. To access the effect of nucleosome turnover on the epigenetic landscape, we investigated H3K79 methylation, which is produced by a single methyltransferase (Dot1l) with no known demethylase. Using chemical-induced proximity (CIP), we find that the valency of H3K79 methylation (mono-, di-, and tri-) is determined by nucleosome turnover rates. Furthermore, propagation of this mark is predicted by nucleosome turnover simulations over the genome and accounts for the asymmetric distribution of H3K79me toward the transcriptional unit. More broadly, a meta-analysis of other conserved histone modifications demonstrates that nucleosome turnover models predict both valency and chromosomal propagation of methylation marks. Based on data from worms, flies, and mice, we propose that the turnover of modified nucleosomes is a general means of propagation of epigenetic marks and a determinant of methylation valence.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Histonas
/
Nucleosomas
/
Genoma
/
Metilación de ADN
/
Epigénesis Genética
/
Células Madre Embrionarias de Ratones
Tipo de estudio:
Health_economic_evaluation
/
Prognostic_studies
/
Systematic_reviews
Límite:
Animals
/
Humans
Idioma:
En
Año:
2019
Tipo del documento:
Article