CPAF, HSP60 and MOMP antigens elicit pro-inflammatory cytokines production in the peripheral blood mononuclear cells from genital Chlamydia trachomatis-infected patients.
Immunobiology
; 224(1): 34-41, 2019 01.
Article
en En
| MEDLINE
| ID: mdl-30477893
ABSTRACT
BACKGROUND:
Persistent inflammation caused by Chlamydia trachomatis in the female genital compartment represents one of the major causes of pelvic inflammatory disease (PID), ectopic pregnancy and infertility in females. Here, we examined the pro-inflammatory cytokine response following stimulation with three different types of C. trachomatis antigens, viz. chlamydial protease-like factor (CPAF), heat shock protein 60 (HSP60) and major outer membrane protein (MOMP).METHODS:
A total of 19 patients with genital C. trachomatis infection and 10 age-matched healthy controls were recruited for the study. Peripheral blood mononuclear cells (PBMCs) isolated from genital C. trachomatis-infected females were cultured in the presence of CPAF, HSP60 and MOMP antigens, and cytokines were measured by ELISA assay.RESULTS:
We reported that pro-inflammatory cytokines (TNF-α, IL-1ß and IL-6) were robustly secreted following antigenic exposure. Notably, CPAP and MOMP were more potent in triggering IL-1ß, as compared to HSP60. Elevated levels of the proinflammatory cytokines were also noted in the samples infected with plasmid-bearing C. trachomatis as compared to those infected with plasmid-free strains.CONCLUSIONS:
Our study highlights distinct ability of chlamydial antigens in triggering pro-inflammatory response in the host immune cells.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Endopeptidasas
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Proteínas de la Membrana Bacteriana Externa
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Leucocitos Mononucleares
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Infecciones por Chlamydia
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Chlamydia trachomatis
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Chaperonina 60
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Genitales
Límite:
Adult
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Female
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Humans
Idioma:
En
Año:
2019
Tipo del documento:
Article