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Faster, sharper, and deeper: structured illumination microscopy for biological imaging.
Wu, Yicong; Shroff, Hari.
  • Wu Y; Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA. yicong.wu@nih.gov.
  • Shroff H; Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA.
Nat Methods ; 15(12): 1011-1019, 2018 12.
Article en En | MEDLINE | ID: mdl-30478322
ABSTRACT
Structured illumination microscopy (SIM) allows rapid, super-resolution (SR) imaging in live specimens. We review recent technical advances in SR-SIM, with emphasis on imaging speed, resolution, and depth. Since its introduction decades ago, the technique has grown to offer myriad implementations, each with its own strengths and weaknesses. We discuss these, aiming to provide a practical guide for biologists and to highlight which approach is best suited to a given application.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Procesamiento de Imagen Asistido por Computador / Iluminación / Microscopía Fluorescente / Modelos Biológicos Límite: Humans Idioma: En Año: 2018 Tipo del documento: Article
Texto completo
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XML
PubMed Links
Search on Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Procesamiento de Imagen Asistido por Computador / Iluminación / Microscopía Fluorescente / Modelos Biológicos Límite: Humans Idioma: En Año: 2018 Tipo del documento: Article