[Comparative analysis of multiple index constituents in Ophiopogonis Radix and Liriopes Radix].
Zhongguo Zhong Yao Za Zhi
; 43(20): 4084-4092, 2018 Oct.
Article
en Zh
| MEDLINE
| ID: mdl-30486535
ABSTRACT
An analytical method based on UFLC-QTRAP-MS/MS was established for simultaneous determination of thirty-three components including steroidal saponins, homoisoflavonoids, amino acids and nucleosides in Ophiopogonis Radix. Thirty-three target components of commercial medicinal materials of Maidong were comparative analysis. Synergi™ Hydro-RP 100î¦ column (2.0 mm × 100 mm, 2.5 µm) was used with 0.1% formic acid solution-0.1% formic acid acetonitrile for gradient elution at a flow rate of 0.4 mL·min⻹. In addition, multiple reaction monitoring (MRM) mode was employed. The data were comprehensively processed and analyzed with hierarchical clustering analysis(HCA), principal component analysis(PCA) and partial least squares discriminant analysis(PLS-DA) methods. All components showed good linearity(r>0.999 0) within the tested ranges. The average recoveries were between 96.23%-102.0%, and the relative standard deviation(RSD) were less than 5%. The results showed that there were significant differences in components between Ophiopogonis Radix and Liriopes Radix, with seven components obviously different. This method was useful for providing basis for the comprehensive evaluation and intrinsic quality control of Ophiopogonis Radix and Liriopes Radix , and may provide a new method reference for the identification of Ophiopogonis Radix and Liriopes Radix.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Saponinas
/
Medicamentos Herbarios Chinos
/
Liriope (Planta)
/
Ophiopogon
Idioma:
Zh
Año:
2018
Tipo del documento:
Article