Granulin 1 Promotes Retinal Regeneration in Zebrafish.
Invest Ophthalmol Vis Sci
; 59(15): 6057-6066, 2018 12 03.
Article
en En
| MEDLINE
| ID: mdl-30577041
ABSTRACT
Purpose:
Retinal degenerative diseases can progress to severe reductions of vision. In general, the changes are permanent in higher vertebrates, including humans; however, retinal regeneration can occur in lower vertebrates, such as amphibians and teleost fish. Progranulin is a secreted growth factor that is involved in normal development and wound-healing processes. We have shown that progranulin promotes the proliferation of retinal precursor cells in mouse retinas. The purpose of this study was to investigate the role played by granulin 1 (grn1) in the retinal regeneration in zebrafish.Methods:
We injured the retina of zebrafish with needle puncturing, and the retinas were examined at different times after the injury. We also checked the proliferation and the expression of retinal regeneration-related genes after knockdown of grn1 by electroporation with morpholino oligonucleotides (MO) and intravitreal injection of recombinant grn1.Results:
Our results showed that the level of grn1 was highly increased after retinal injury, and it was expressed in various types of retinal cells. A knockdown of grn1 reduced the proliferation of Müller glial cells in zebrafish eyes undergoing retinal regeneration. The knockdown of grn1 also reduced the expression of achaete-scute homolog 1a (ascl1a), an important factor in retinal regeneration. An intravitreal injection of recombinant grn1 led to a proliferation of Müller glial cells and an increase in the expression of retinal regeneration-related genes, such as ascl1a and lin28.Conclusions:
These findings suggested that grn1 should be considered as a target for stimulating the dedifferentiation of Müller glial cells and retinal regeneration.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Regeneración
/
Retina
/
Degeneración Retiniana
/
Proteínas de Pez Cebra
/
Granulinas
Tipo de estudio:
Etiology_studies
Límite:
Animals
Idioma:
En
Año:
2018
Tipo del documento:
Article