[Rapid analysis on chemical constituents in Qige Keli by RRLC-Q-TOF-MS/MS].
Zhongguo Zhong Yao Za Zhi
; 43(21): 4240-4247, 2018 Nov.
Article
en Zh
| MEDLINE
| ID: mdl-30583624
ABSTRACT
The qualitative analysis method of RRLC-Q-TOF-MS/MS was established for determine the chemical constituents in Qige Keli. Kramosil C18 column (4.6 mm×150 mm, 3.5 µm) was used at the temperature of 30 °C. The mobile phase was 0.2% formic acid and acetonitrile by gradient elution, with a flow rate at 1.0 mL·min⻹, and the injection volume was 10 µL. The high-resolution quadrupole time-flight mass spectrometry was used as detector with electrospray ion source in both positive and negative models. On the basis of medicinal materials, reference materials, literature reports, and mass spectrometry data, the chemical composition in the Qige Keli was identified. A total of 44 compounds were identified, including 3 flavonoids, 21 flavonoid glycosides, 8 organic acids, 6 lactones, and 3 saponins. The results laid the foundation for the quality control of Qige Keli and the further research on pharmacodynamic materials.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Medicamentos Herbarios Chinos
/
Fitoquímicos
Tipo de estudio:
Qualitative_research
Idioma:
Zh
Año:
2018
Tipo del documento:
Article