Selective ß-N-acetylhexosaminidase from Aspergillus versicolor-a tool for producing bioactive carbohydrates.

Bojarová, Pavla; Kulik, Natallia; Slámová, Kristýna; Hubálek, Martin; Kotik, Michael; Cvacka, Josef; Pelantová, Helena; Kren, Vladimír

Bojarová, Pavla; Kulik, Natallia; Slámová, Kristýna; Hubálek, Martin; Kotik, Michael; Cvacka, Josef; Pelantová, Helena; Kren, Vladimír.

Bojarová P; Laboratory of Biotransformation, Institute of Microbiology, Czech Academy of Sciences, Vídenská 1083, CZ-14220, Prague 4, Czech Republic. bojarova@biomed.cas.cz.
Kulik N; Laboratory of Structure and Function of Proteins, Institute of Microbiology, Czech Academy of Sciences, Zámek 136, CZ-37333, Nové Hrady, Czech Republic.
Slámová K; Laboratory of Biotransformation, Institute of Microbiology, Czech Academy of Sciences, Vídenská 1083, CZ-14220, Prague 4, Czech Republic.
Hubálek M; Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Flemingovo námestí 2, CZ-16610, Prague 6, Czech Republic.
Kotik M; Laboratory of Biotransformation, Institute of Microbiology, Czech Academy of Sciences, Vídenská 1083, CZ-14220, Prague 4, Czech Republic.
Cvacka J; Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Flemingovo námestí 2, CZ-16610, Prague 6, Czech Republic.
Pelantová H; Laboratory of Biotransformation, Institute of Microbiology, Czech Academy of Sciences, Vídenská 1083, CZ-14220, Prague 4, Czech Republic.
Kren V; Laboratory of Biotransformation, Institute of Microbiology, Czech Academy of Sciences, Vídenská 1083, CZ-14220, Prague 4, Czech Republic.

Appl Microbiol Biotechnol ; 103(4): 1737-1753, 2019 Feb.

Article en En | MEDLINE | ID: mdl-30603849

Asunto(s)

Aspergillus/enzimología; Disacáridos/metabolismo; Proteínas Recombinantes/metabolismo; Talaromyces/enzimología; beta-N-Acetilhexosaminidasas/metabolismo; Dominio Catalítico; Cromatografía por Intercambio Iónico; Expresión Génica; Modelos Moleculares; Pichia/genética; Pichia/metabolismo; Conformación Proteica; Proteínas Recombinantes/química; Proteínas Recombinantes/genética; Proteínas Recombinantes/aislamiento & purificación; beta-N-Acetilhexosaminidasas/química; beta-N-Acetilhexosaminidasas/genética; beta-N-Acetilhexosaminidasas/aislamiento & purificación

Palabras clave

Aspergillus versicolor; Glycosidase; Heterologous expression; Homology modeling; Pichia pastoris; ß-N-Acetylhexosaminidase

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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Aspergillus / Beta-N-Acetilhexosaminidasas / Proteínas Recombinantes / Talaromyces / Disacáridos Idioma: En Año: 2019 Tipo del documento: Article

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