[The influence of metformin on age-related changes in the number and proliferation of dermal fibroblasts in mice.]

The goal of our work was to examine the effects of metformin on age-related changes in the number and proliferation of dermal fibroblasts in mice. The study of the dermis was carried out at five months' mice that received drinking water with metformin at concentrations 500 mg/l from two months (within 90 days). Five months' mice received drinking water without metformin and they were as a control. Material of two months' mice was also used in the work. We counted the total number of dermal fibroblasts and lobe of fibroblasts with positive coloration on Ki-67. The results showed that there has been a decrease in the total number of dermal fibroblasts by 42,3% from two months of mouse's life up to five months and lobe of fibroblasts with positive coloration on Ki-67 by 12%. The total number of dermal fibroblasts in five months' mice was lower for 13,4%by using metformin. The lobe of fibroblasts with positive coloration on Ki-67 was reduced by 27,3% in comparison with information of animals that have not received metformin. Thus, age-related reduction of dermal fibroblasts in mice is due to decline in their proliferative activity. Metformin has an inhibited impact on proliferation of dermal fibroblasts in mice.