Genetically Encoded Cholesterol-Modified Polypeptides.
J Am Chem Soc
; 141(2): 945-951, 2019 01 16.
Article
en En
| MEDLINE
| ID: mdl-30608674
ABSTRACT
Biological systems use post-translational modifications (PTMs) to control the structure, location, and function of proteins after expression. Despite the ubiquity of PTMs in biology, their use to create genetically encoded recombinant biomaterials is limited. We have utilized a natural lipidation PTM (hedgehog-mediated cholesterol modification of proteins) to create a class of hybrid biomaterials called cholesterol-modified polypeptides (CHaMPs) that exhibit programmable self-assembly at the nanoscale. To demonstrate the biomedical utility of CHaMPs, we used this approach to append cholesterol to biologically active peptide exendin-4 that is an approved drug for the treatment of type II diabetes. The exendin-cholesterol conjugate self-assembled into micelles, and these micelles activate the glucagon-like peptide-1 receptor with a potency comparable to that of current gold standard treatments.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Procesamiento Proteico-Postraduccional
/
Colesterol
/
Exenatida
Límite:
Animals
/
Humans
Idioma:
En
Año:
2019
Tipo del documento:
Article