Time-lapse imaging of Ca2+-induced swelling and permeability transition: Single mitochondrion study.
Arch Biochem Biophys
; 663: 288-296, 2019 03 15.
Article
en En
| MEDLINE
| ID: mdl-30659803
ABSTRACT
Mitochondrial functions are closely related to the membrane structure. Mitochondrial swelling, which is accompanied with dissipation of the crista structure and rupture of the outer membrane, have been observed as mitochondrial damage when mitochondria are under Ca2+-overload or oxidative stress. Although these phenomena have been well studied, the detailed behaviors of individual mitochondria upon swelling remain unknown. The aim of this study was to investigate the detailed behavior of mitochondrial volume upon addition of Ca2+. Here, we report for the first time, time-lapse measurements of single mitochondrion swelling and permeability transition induced by Ca2+ by optical microscopy. We added 220⯵M Ca2+ to mitochondria, and found that 1) the swelling rate depended on the mitochondrion, 2) a small number of mitochondria showed step-like swelling, 3) cyclosporin A decreased the percentage of mitochondria that underwent swelling induced by Ca2+, but did not affect the amplitude of swelling, 4) permeability transition is necessary but not sufficient for Ca2+-induced swelling, 5) permeability transition is more sensitive to Ca2+ than swelling, 6) Ca2+ stimulated mitochondrial swelling after permeability transition. These results suggest that single mitochondrion measurement of swelling is a powerful tool for examining the regulation of mitochondrial structure.
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Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Calcio
/
Imagen de Lapso de Tiempo
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Dilatación Mitocondrial
Límite:
Animals
Idioma:
En
Año:
2019
Tipo del documento:
Article