Expression of the human apolipoprotein AI gene fused to the E. coli gene for beta-galactosidase.
FEBS Lett
; 194(2): 343-6, 1986 Jan 06.
Article
en En
| MEDLINE
| ID: mdl-3079712
ABSTRACT
The human apoAI gene was expressed in E. coli by in-frame fusion to a modified beta-galactosidase gene present in plasmid pUR291. The fused beta-galactosidase-apoAI gene product was expressed at a high level and was recognized by an anti-human apoAI antiserum. Besides the fused protein, at least one degradation product having an Mr similar to that of beta-galactosidase was present in high amounts in bacterial extracts. These results and those of a pulse-chase experiment indicate that degradation took place only in the apoAI moiety of the chimeric protein.
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Banco de datos:
MEDLINE
Asunto principal:
Apolipoproteínas A
/
Genes Reguladores
/
Beta-Galactosidasa
/
Escherichia coli
/
Galactosidasas
/
Lipoproteínas HDL
Límite:
Humans
Idioma:
En
Año:
1986
Tipo del documento:
Article