Nuclear speckle fusion via long-range directional motion regulates speckle morphology after transcriptional inhibition.
J Cell Sci
; 132(8)2019 04 17.
Article
en En
| MEDLINE
| ID: mdl-30858197
ABSTRACT
Although the formation of RNA-protein bodies has been studied intensively, their mobility and how their number and size are regulated are still poorly understood. Here, we show significantly increased mobility of nuclear speckles after transcriptional inhibition, including long-range directed motion of one speckle towards another speckle, terminated by speckle fusion, over distances up to 4â
µm and with velocities between 0.2â
µm/min and 1.5â
µm/min. Frequently, three or even four speckles follow very similar paths, with new speckles appearing along the path followed by a preceding speckle. Speckle movements and fusion events contribute to fewer, but larger, speckles after transcriptional inhibition. These speckle movements are not actin dependent, but occur within chromatin-depleted channels enriched with small granules containing the speckle marker protein SON. Similar long-range speckle movements and fusion events were observed after heat shock or heavy metal stress, and during late G2 and early prophase. Our observations suggest a mechanism for long-range, directional nuclear speckle movements, contributing to overall regulation of nuclear speckle number and size as well as overall nuclear organization. This article has an associated First Person interview with the first author of the paper.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Activación Transcripcional
/
Transgenes
/
Respuesta al Choque Térmico
/
Cuerpos de Inclusión Intranucleares
Límite:
Animals
/
Humans
Idioma:
En
Año:
2019
Tipo del documento:
Article