Chemical structure of 3-carbethoxypsoralen-DNA photoadducts.
Biochimie
; 68(6): 787-95, 1986 Jun.
Article
en En
| MEDLINE
| ID: mdl-3092876
ABSTRACT
The enzymatic digest from salmon sperm DNA photochemically modified by the monofunctional 3-carbethoxypsoralen was analyzed by high-performance liquid chromatography. The modified nucleosides extracted from DNA were compared with model compounds obtained from irradiation in the dry state of mixtures of 3-carbethoxypsoralen with 2'-deoxyribonucleosides whose chemical structures had previously been characterized. The main photoadducts formed in DNA are two cis-syn diastereoisomers formed via a C4-cycloaddition reaction involving the 4', 5' double bond of 3-carbethoxypsoralen and the 5,6 double bond of 2'-deoxythymidine. Among them, the most polar one accounts for 72%. Under the same conditions, photoadducts formed between 3-carbethoxypsoralen and 2'deoxycytidine account for less than 1%.
Search on Google
Banco de datos:
MEDLINE
Asunto principal:
Furocumarinas
/
ADN
/
Luz
Idioma:
En
Año:
1986
Tipo del documento:
Article