Conversion of random X-inactivation to imprinted X-inactivation by maternal PRC2.
Elife
; 82019 04 02.
Article
en En
| MEDLINE
| ID: mdl-30938678
ABSTRACT
Imprinted X-inactivation silences genes exclusively on the paternally-inherited X-chromosome and is a paradigm of transgenerational epigenetic inheritance in mammals. Here, we test the role of maternal vs. zygotic Polycomb repressive complex 2 (PRC2) protein EED in orchestrating imprinted X-inactivation in mouse embryos. In maternal-null (Eedm-/-) but not zygotic-null (Eed-/-) early embryos, the maternal X-chromosome ectopically induced Xist and underwent inactivation. Eedm-/- females subsequently stochastically silenced Xist from one of the two X-chromosomes and displayed random X-inactivation. This effect was exacerbated in embryos lacking both maternal and zygotic EED (Eedmz-/-), suggesting that zygotic EED can also contribute to the onset of imprinted X-inactivation. Xist expression dynamics in Eedm-/- embryos resemble that of early human embryos, which lack oocyte-derived maternal PRC2 and only undergo random X-inactivation. Thus, expression of PRC2 in the oocyte and transmission of the gene products to the embryo may dictate the occurrence of imprinted X-inactivation in mammals.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Inactivación del Cromosoma X
/
Complejo Represivo Polycomb 2
/
Ratones
Límite:
Animals
Idioma:
En
Año:
2019
Tipo del documento:
Article