Effects of dietary mixed probiotics on growth, non-specific immunity, intestinal morphology and microbiota of juvenile pacific white shrimp, Litopenaeus vannamei.
Fish Shellfish Immunol
; 90: 456-465, 2019 Jul.
Article
en En
| MEDLINE
| ID: mdl-31075403
ABSTRACT
This study was conducted to elucidate the effects of dietary mixed probiotics on growth, non-specific immunity, intestinal morphology and microbiota of juvenile pacific white shrimp, Litopenaeus vannamei. Juvenile shrimp (initial body weight 1.21⯱â¯0.01â¯g) were fed diets containing graded probiotics (F1 0â¯mg/kg probiotics; F2 1000â¯mg/kg probiotics; F3 2000â¯mg/kg probiotics; F4 4000â¯mg/kg compound probiotics; F5 6000â¯mg/kg probiotics; F6 8000â¯mg/kg probiotics) for 8 weeks. The result of this trial showed that the growth performance (SGR, WG, FBW) of shrimp fed diets containing probiotics (F2â¼F6) were significantly higher than that of shrimp fed diet without supplemental probiotics (F1) (Pâ¯<â¯0.05), and the highest values of the growth performance (SGR, WG, FBW) and lowest FCR were found in shrimp fed the diet containing 2000â¯mg/kg probiotics. Total antioxidant capacity of shrimp fed diet F2 and F3 were significantly higher than that of shrimp fed the basal diets (Pâ¯<â¯0.05). Superoxide dismutase in F4 treatment was significantly higher than that of basal treatment (Pâ¯<â¯0.05). Catalase of shrimp in all probiotics supplemented (F2â¼F6) treatments were significantly higher than that of the control one (F1) (Pâ¯<â¯0.05). Malondialdehyde in F5 groups was significantly lower than that of F1 groups (Pâ¯<â¯0.05). Alkline phosphatase and acid phosphatase in F3 treatments were significantly higher than those of the basal one (Pâ¯<â¯0.05). Lysozyme of shrimp fed F2â¼F6 were significantly higher than that of shrimp fed F1 diet (Pâ¯<â¯0.05). The lipase and amylase activities in 2000â¯mg/kg probiotics groups showed the highest activities and were significantly higher than that of control one (Pâ¯<â¯0.05). Intestinal villi height in F3â¼F6 treatments were significantly higher than that of control one (Pâ¯<â¯0.05). Alpha diversity indices including observed species, chao1, ACE and shannon indices showed that F2 and F3 groups had higher microbial diversity in their intestines, both richness and evenness. PCA plot showed that there was a clear shift of F2 and F3 groups from the control groups in microbial community structure. The dominant phyla in pacific white shrimp are proteobacteria, bacteroidetes and actinobacteria, the dominant genus were algoriphagus and vibrio. As the probiotics increased, the gemmatimonadetes, acidobacteria, deltaproteobacteria and xanthomonadales firstly increased and then decreased, with the highest content in F2 group, which was no significant difference to F3 group (Pâ¯>â¯0.05) while significantly higher than other groups (Pâ¯<â¯0.05). In conclusion, the supplement of mixed species probiotics can promote growth performance, enhance the non-specific immunity, influence the microbiota of the pacific white shrimps and the recommended optimum dosage in diet of Litopenaeus vannamei was 2000â¯mg/kg.
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Texto completo:
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Banco de datos:
MEDLINE
Asunto principal:
Probióticos
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Penaeidae
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Microbioma Gastrointestinal
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Inmunidad Innata
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Intestinos
Límite:
Animals
Idioma:
En
Año:
2019
Tipo del documento:
Article