A novel short chain dehydrogenase from Bacillus megaterium for the conversion of the sesquiterpene nootkatol to (+)-nootkatone.
J Biotechnol
; 301: 52-55, 2019 Aug 10.
Article
en En
| MEDLINE
| ID: mdl-31150680
ABSTRACT
(+)-Nootkatone is a natural ingredient that occurs in grapefruit and certain other plants and is responsible for the characteristic smell of grapefruit. Due to its versatile applications in the flavor and fragrance industry as well as its application in some medical uses it recruits the interests of academic research along with industrial biotechnology. In the current work we present the application of a novel short chain dehydrogenase from Bacillus megaterium in an in vivo whole-cell biocatalyst system for the conversion of the intermediate nootkatol into the industrially valuable (+)-nootkatone. The newly identified dehydrogenase converted nootkatol selectively and efficiently into the final product. The conversion ratio of about 100% was achieved within 40â¯min yielding about 44â¯mg/L (+)-nootkatone. Furthermore, the herein identified dehydrogenase provides a new tool to overcome the limitation of the two-step enzymatic biotechnological process for the production of (+)-nootkatone.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Sesquiterpenos
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Bacillus megaterium
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Proteínas Bacterianas
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Deshidrogenasas-Reductasas de Cadena Corta
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Sesquiterpenos Policíclicos
Tipo de estudio:
Prognostic_studies
Idioma:
En
Año:
2019
Tipo del documento:
Article