MicroRNA106b functions as an oncogene and regulates tumor viability and metastasis by targeting LARP4B in prostate cancer.
Mol Med Rep
; 20(2): 951-958, 2019 Aug.
Article
en En
| MEDLINE
| ID: mdl-31173237
ABSTRACT
Prostate cancer (PCa) is the most common malignancy among males worldwide, and is one of the leading causes of cancerrelated mortality. MicroRNAs (miRs) are a type of endogenous, noncoding RNA that serve a key role in pathological processes, and have been demonstrated to be involved in the formation and progression of PCa. Previous studies have reported that miR106b acts as an oncogene; however, the specific effects of miR106b on PCa have not been fully elucidated. The present study aimed to investigate the role and underlying molecular mechanisms of miR106b in the initiation and progression of PCa. In this study, miR106b was reported to be overexpressed and larelated protein 4B (LARP4B) was downregulated in PCa tissues compared with paracancerous tissues. In addition, LARP4B was identified as a target gene of miR106b by bioinformatics prediction analysis and a dual luciferase reporter gene assay. Furthermore, MTT, wound healing and Transwell assays were performed to evaluate PCa cell viability, and migration and invasive abilities. The data revealed that inhibition of miR106b significantly suppressed the viability, migration and invasion of PCa cells. In addition, inhibition of miR106b significantly suppressed the mRNA and protein expression of cancerrelated genes, including matrix metalloproteinase2, cluster of differentiation 44 and Ki67, and increased that of the tumor suppressor, mothers against decapentaplegic homolog 2. Collectively, the findings of the present study indicated that miR106b may target LAR4B to inhibit cancer cell viability, migration and invasion, and may be considered as a novel therapeutic target in PCa.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Próstata
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Neoplasias de la Próstata
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Ribonucleoproteínas
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Autoantígenos
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Regulación Neoplásica de la Expresión Génica
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MicroARNs
Tipo de estudio:
Prognostic_studies
Límite:
Aged
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Humans
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Male
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Middle aged
Idioma:
En
Año:
2019
Tipo del documento:
Article