Young seedlings adapt to stress by retaining starch and retarding growth through ABA-Dependent and -independent pathways in Arabidopsis.

ABSTRACT

The metabolism of starch, the major resource of carbohydrates and an essential determinant of plant fitness in plants, often exhibits plasticity in response to challenging environmental conditions. Previous study of leaf starch turnover under abiotic stresses documented conflicting patterns. To understand this discrepancy and examine the process in more detail, we grew Arabidopsis seedlings under a series of typical stress conditions, observed and quantified leaf starch content at different time. By electron microscope, iodine staining and starch quantification, we confirm that short-term (<8 h) stress treatments, such as osmotic stress, high salinity, caused rapid starch decrease during the night while long-term (>56 h) stresses increased starch content significantly. Exogenous abscisic acid (ABA) induced similar effects to the abiotic stresses. Comparison of the starch content in wild type (WT) and the ABA receptor quadruple mutant pyr1;pyl1;pyl2;pyl4 suggests that the stress-induced starch turnover change is also mediated by ABA-independent pathways. In addition, more energy supply caused severer growth defect under stress conditions. And the transcription levels of both starch biosynthesis enzymes (APL1 and APL3) and starch degradation enzymes (SEX1, SEX4, BAM1 and BAM3) exhibited differential increase under long-term stresses. Taken together, our data strongly suggest that young seedlings adapt to stress by retaining starch and retarding growth through both ABA-dependent and -independent pathways.