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Coherent diffractive imaging of microtubules using an X-ray laser.
Brändén, Gisela; Hammarin, Greger; Harimoorthy, Rajiv; Johansson, Alexander; Arnlund, David; Malmerberg, Erik; Barty, Anton; Tångefjord, Stefan; Berntsen, Peter; DePonte, Daniel P; Seuring, Carolin; White, Thomas A; Stellato, Francesco; Bean, Richard; Beyerlein, Kenneth R; Chavas, Leonard M G; Fleckenstein, Holger; Gati, Cornelius; Ghoshdastider, Umesh; Gumprecht, Lars; Oberthür, Dominik; Popp, David; Seibert, Marvin; Tilp, Thomas; Messerschmidt, Marc; Williams, Garth J; Loh, N Duane; Chapman, Henry N; Zwart, Peter; Liang, Mengning; Boutet, Sébastien; Robinson, Robert C; Neutze, Richard.
  • Brändén G; Department of Chemistry and Molecular Biology, University of Gothenburg, Box 462, 40530, Gothenburg, Sweden. gisela.branden@gu.se.
  • Hammarin G; Department of Chemistry and Molecular Biology, University of Gothenburg, Box 462, 40530, Gothenburg, Sweden.
  • Harimoorthy R; Department of Chemistry and Molecular Biology, University of Gothenburg, Box 462, 40530, Gothenburg, Sweden.
  • Johansson A; Department of Chemistry and Molecular Biology, University of Gothenburg, Box 462, 40530, Gothenburg, Sweden.
  • Arnlund D; Department of Chemistry and Molecular Biology, University of Gothenburg, Box 462, 40530, Gothenburg, Sweden.
  • Malmerberg E; Molecular Biophysics and Integrated Bio-Imaging Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Rd, 94720, Berkeley, CA, USA.
  • Barty A; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, 22607, Hamburg, Germany.
  • Tångefjord S; Department of Chemistry and Molecular Biology, University of Gothenburg, Box 462, 40530, Gothenburg, Sweden.
  • Berntsen P; Department of Chemistry and Molecular Biology, University of Gothenburg, Box 462, 40530, Gothenburg, Sweden.
  • DePonte DP; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA, USA.
  • Seuring C; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, 22607, Hamburg, Germany.
  • White TA; The Hamburg Center for Ultrafast Imaging, 22761, Hamburg, Germany.
  • Stellato F; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, 22607, Hamburg, Germany.
  • Bean R; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, 22607, Hamburg, Germany.
  • Beyerlein KR; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, 22607, Hamburg, Germany.
  • Chavas LMG; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, 22607, Hamburg, Germany.
  • Fleckenstein H; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, 22607, Hamburg, Germany.
  • Gati C; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, 22607, Hamburg, Germany.
  • Ghoshdastider U; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, 22607, Hamburg, Germany.
  • Gumprecht L; Institute of Molecular and Cell Biology, Biopolis, A*STAR (Agency for Science, Technology and Research), 138673, Singapore, Singapore.
  • Oberthür D; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, 22607, Hamburg, Germany.
  • Popp D; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, 22607, Hamburg, Germany.
  • Seibert M; Institute of Molecular and Cell Biology, Biopolis, A*STAR (Agency for Science, Technology and Research), 138673, Singapore, Singapore.
  • Tilp T; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA, USA.
  • Messerschmidt M; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, 22607, Hamburg, Germany.
  • Williams GJ; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA, USA.
  • Loh ND; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA, USA.
  • Chapman HN; Department of Physics, National University of Singapore, 117551, Singapore, Singapore.
  • Zwart P; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, 22607, Hamburg, Germany.
  • Liang M; The Hamburg Center for Ultrafast Imaging, 22761, Hamburg, Germany.
  • Boutet S; Department of Physics, University of Hamburg, 22761, Hamburg, Germany.
  • Robinson RC; Molecular Biophysics and Integrated Bio-Imaging Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Rd, 94720, Berkeley, CA, USA.
  • Neutze R; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, 22607, Hamburg, Germany.
Nat Commun ; 10(1): 2589, 2019 06 13.
Article en En | MEDLINE | ID: mdl-31197138
ABSTRACT
X-ray free electron lasers (XFELs) create new possibilities for structural studies of biological objects that extend beyond what is possible with synchrotron radiation. Serial femtosecond crystallography has allowed high-resolution structures to be determined from micro-meter sized crystals, whereas single particle coherent X-ray imaging requires development to extend the resolution beyond a few tens of nanometers. Here we describe an intermediate

approach:

the XFEL imaging of biological assemblies with helical symmetry. We collected X-ray scattering images from samples of microtubules injected across an XFEL beam using a liquid microjet, sorted these images into class averages, merged these data into a diffraction pattern extending to 2 nm resolution, and reconstructed these data into a projection image of the microtubule. Details such as the 4 nm tubulin monomer became visible in this reconstruction. These results illustrate the potential of single-molecule X-ray imaging of biological assembles with helical symmetry at room temperature.
Asunto(s)

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Tubulina (Proteína) / Electrones / Imagen Molecular / Rayos Láser / Microtúbulos Idioma: En Año: 2019 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Tubulina (Proteína) / Electrones / Imagen Molecular / Rayos Láser / Microtúbulos Idioma: En Año: 2019 Tipo del documento: Article