Evidences of plasma membrane-mediated ROS generation upon ELF exposure in neuroblastoma cells supported by a computational multiscale approach.
Biochim Biophys Acta Biomembr
; 1861(8): 1446-1457, 2019 08 01.
Article
en En
| MEDLINE
| ID: mdl-31199897
ABSTRACT
BACKGROUND:
Molecular mechanisms of interaction between cells and extremely low frequency magnetic fields (ELF-MFs) still represent a matter of scientific debate. In this paper, to identify the possible primary source of oxidative stress induced by ELF-MF in SH-SY5Y human neuroblastoma cells, we estimated the induced electric field and current density at the cell level.METHODS:
We followed a computational multiscale approach, estimating the local electric field and current density from the whole sample down to the single cell level. The procedure takes into account morphological modeling of SH-SY5Y cells, arranged in different topologies. Experimental validation has been carried out neuroblastoma cells have been treated with Diphenyleneiodonium (DPI) -an inhibitor of the plasma membrane enzyme NADPH oxidase (Nox)- administered 24â¯h before exposure to 50â¯Hz (1 mT) MF.RESULTS:
Macroscopic and microscopic dosimetric evaluations suggest that increased current densities are induced at the plasma membrane/extra-cellular medium interface; identifying the plasma membrane as the main site of the ELF-neuroblastoma cell interaction. The in vitro results provide an experimental proof that plasma membrane Nox exerts a key role in the redox imbalance elicited by ELF, as DPI treatment reverts the generation of reactive oxygen species induced by ELF exposure. GENERALSIGNIFICANCE:
Microscopic current densities induced at the plasma membrane are likely to play an active physical role in eliciting ELF effects related to redox imbalance. Multiscale computational dosimetry, supported by an in vitro approach for validation, is proposed as the innovative and rigorous paradigm to unveil mechanisms underlying the complex ELF-MF interactions.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Membrana Celular
/
Especies Reactivas de Oxígeno
/
Campos Electromagnéticos
/
Neuroblastoma
Límite:
Humans
Idioma:
En
Año:
2019
Tipo del documento:
Article