A Novel Pancreatic Imaging Window for Stabilized Longitudinal In Vivo Observation of Pancreatic Islets in Murine Model.
Diabetes Metab J
; 44(1): 193-198, 2020 02.
Article
en En
| MEDLINE
| ID: mdl-31237131
ABSTRACT
Longitudinal imaging of murine pancreas is technically challenging due to the mechanical softness of the tissue influenced by peristalsis. Here, we report a novel pancreatic imaging window for long-term stabilized cellular-level observation of the islets in the pancreas in vivo. By spatially separating the pancreas from the bowel movement and physiologic respiration with a metal plate integrated in the imaging window, we successfully tracked the pancreatic islets up to three weeks and visualized the dumbbell-shape transformation from the single islet. This window can be a useful tool for long-term cellular-level visualization of the microstructure in the pancreas.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Páncreas
/
Islotes Pancreáticos
/
Microscopía Intravital
Límite:
Animals
Idioma:
En
Año:
2020
Tipo del documento:
Article