Immunoaffinity chromatography of bovine FSH using monoclonal antibodies.
J Endocrinol
; 115(2): 283-8, 1987 Nov.
Article
en En
| MEDLINE
| ID: mdl-3125301
ABSTRACT
Bovine FSH (bFSH) was used to immunize BALB/c mice. Spleen cells were fused to the SP 2/0 cell line to produce hybridomas that secreted monoclonal antibodies to bFSH. One of these antibodies (USDA-bFSH-MC28) was extensively characterized and found to be a gamma 1 with kappa light chains, having extremely low cross-reactivity with other bovine pituitary hormones and with ovine and porcine FSH. The dissociation constant as measured by Scatchard analysis was 4.3 nmol/l, and proved to be in a very useful range for affinity chromatography. In an essentially one-step immunoaffinity chromatography procedure, bFSH was easily isolated in a single chromatographic step from crude anterior pituitary homogenate with better yield and with the same purity as classical chromatographic techniques.
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Banco de datos:
MEDLINE
Asunto principal:
Hormona Folículo Estimulante
/
Anticuerpos Monoclonales
Límite:
Animals
Idioma:
En
Año:
1987
Tipo del documento:
Article