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Zika virus differentially infects human neural progenitor cells according to their state of differentiation and dysregulates neurogenesis through the Notch pathway.
Ferraris, Pauline; Cochet, Marielle; Hamel, Rodolphe; Gladwyn-Ng, Ivan; Alfano, Christian; Diop, Fodé; Garcia, Déborah; Talignani, Loïc; Montero-Menei, Claudia N; Nougairède, Antoine; Yssel, Hans; Nguyen, Laurent; Coulpier, Muriel; Missé, Dorothée.
  • Ferraris P; a MIVEGEC, IRD , Univ. Montpellier, CNRS , Montpellier , France.
  • Cochet M; b UMR1161 Virologie, ANSES, INRA, Ecole Nationale Vétérinaire d'Alfort , Université Paris-Est , Maisons-Alfort , France.
  • Hamel R; a MIVEGEC, IRD , Univ. Montpellier, CNRS , Montpellier , France.
  • Gladwyn-Ng I; c GIGA-Neuroscience, Interdisciplinary Cluster for Applied Genoproteomics (GIGA-R) , University of Liège, C.H.U. Sart Tilman , Liège , Belgium.
  • Alfano C; c GIGA-Neuroscience, Interdisciplinary Cluster for Applied Genoproteomics (GIGA-R) , University of Liège, C.H.U. Sart Tilman , Liège , Belgium.
  • Diop F; a MIVEGEC, IRD , Univ. Montpellier, CNRS , Montpellier , France.
  • Garcia D; a MIVEGEC, IRD , Univ. Montpellier, CNRS , Montpellier , France.
  • Talignani L; a MIVEGEC, IRD , Univ. Montpellier, CNRS , Montpellier , France.
  • Montero-Menei CN; d CRCINA, INSERM , Université de Nantes, Université d'Angers , Angers , France.
  • Nougairède A; e UVE, Aix Marseille Univ-IRD 190 , Inserm 1207-IHU Méditerranée Infection , Marseille , France.
  • Yssel H; f Centre d'Immunologie et des Maladies Infectieuses, Inserm, U1135 , Sorbonne Universités, UPMC, APHP Hôpital Pitié-Salpêtrière , Paris , France.
  • Nguyen L; c GIGA-Neuroscience, Interdisciplinary Cluster for Applied Genoproteomics (GIGA-R) , University of Liège, C.H.U. Sart Tilman , Liège , Belgium.
  • Coulpier M; b UMR1161 Virologie, ANSES, INRA, Ecole Nationale Vétérinaire d'Alfort , Université Paris-Est , Maisons-Alfort , France.
  • Missé D; a MIVEGEC, IRD , Univ. Montpellier, CNRS , Montpellier , France.
Emerg Microbes Infect ; 8(1): 1003-1016, 2019.
Article en En | MEDLINE | ID: mdl-31282298
ABSTRACT
Zika virus (ZIKV) is a mosquito-borne Flavivirus that causes Zika disease with particular neurological complications, including Guillain-Barré Syndrome and congenital microcephaly. Although ZIKV has been shown to directly infect human neural progenitor cells (hNPCs), thereby decreasing their viability and growth, it is as yet unknown which of the cellular pathways involved in the disruption of neurogenesis are affected following ZIKV infection. By comparing the effect of two ZIKV strains in vitro on hNPCs, the differentiation process of the latter cells was found to lead to a decreased susceptibility to infection and cell death induced by each of the ZIKV strains, which was associated with an earlier and stronger antiviral innate immune response in infected, differentiated hNPCs, as compared to undifferentiated cells. Moreover, ZIKV modulated, both in hNPCs and in vivo in fetal brain in an experimental mouse model, the expression of the Notch pathway which is involved in cellular proliferation, apoptosis and differentiation during neurogenesis. These results show that the differentiation state of hNPCs is a significant factor contributing to the outcome of ZIKV infection and furthermore suggest that ZIKV infection might initiate early activation of the Notch pathway resulting in an abnormal differentiation process, implicated in ZIKV-induced brain injury.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Receptor Notch1 / Neurogénesis / Células-Madre Neurales / Virus Zika / Infección por el Virus Zika Tipo de estudio: Prognostic_studies Límite: Animals / Female / Humans Idioma: En Año: 2019 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Receptor Notch1 / Neurogénesis / Células-Madre Neurales / Virus Zika / Infección por el Virus Zika Tipo de estudio: Prognostic_studies Límite: Animals / Female / Humans Idioma: En Año: 2019 Tipo del documento: Article