Miniaturized microscope with flexible light source input for neuronal imaging and manipulation in freely behaving animals.
Biochem Biophys Res Commun
; 517(3): 520-524, 2019 09 24.
Article
en En
| MEDLINE
| ID: mdl-31376934
ABSTRACT
Simultaneous imaging and manipulation of a genetically defined neuronal population can provide a causal link between its activity and function. Here, we designed a miniaturized microscope (or 'miniscope') that allows fluorescence imaging and optogenetic manipulation at the cellular level in freely behaving animals. This miniscope has an integrated optical connector that accepts any combination of external light sources, allowing flexibility in the choice of sensors and manipulators. Moreover, due to its simple structure and use of open source software, the miniscope is easy to build and modify. Using this miniscope, we demonstrate the optogenetic silencing of hippocampal CA1 neurons using two laser light sources-one stimulating a calcium sensor (i.e., jGCaAMP7c) and the other serving as an optogenetic silencer (i.e., Jaws). This new miniscope can contribute to efforts to determine causal relationships between neuronal network dynamics and animal behavior.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Región CA1 Hipocampal
/
Neuroimagen
/
Optogenética
/
Microscopía
/
Red Nerviosa
/
Neuronas
Límite:
Animals
Idioma:
En
Año:
2019
Tipo del documento:
Article