Your browser doesn't support javascript.
loading
Mechanism of cadmium poisoning on testicular injury in mice.
Ren, Yaping; Shao, Wenhua; Zuo, Lijun; Zhao, Wei; Qin, Haizhang; Hua, Yingjie; Lu, Dejie; Mi, Chao; Zeng, Sien; Zu, Liao.
  • Ren Y; Department of Histology and Embryology, Guilin Medical University, Guilin, Guangxi 541004, P.R. China.
  • Shao W; Laboratory of Tumor Immunology and Microenvironmental Regulation, Guilin Medical University, Guilin, Guangxi 541004, P.R. China.
  • Zuo L; Department of Pathology and Physiopathology, Guilin Medical University, Guilin, Guangxi 541004, P.R. China.
  • Zhao W; Department of Histology and Embryology, Guilin Medical University, Guilin, Guangxi 541004, P.R. China.
  • Qin H; Department of Histology and Embryology, Guilin Medical University, Guilin, Guangxi 541004, P.R. China.
  • Hua Y; Department of Histology and Embryology, Guilin Medical University, Guilin, Guangxi 541004, P.R. China.
  • Lu D; Department of Histology and Embryology, Guilin Medical University, Guilin, Guangxi 541004, P.R. China.
  • Mi C; Department of Histology and Embryology, Guilin Medical University, Guilin, Guangxi 541004, P.R. China.
  • Zeng S; Department of Pathology and Physiopathology, Guilin Medical University, Guilin, Guangxi 541004, P.R. China.
  • Zu L; Laboratory of Tumor Immunology and Microenvironmental Regulation, Guilin Medical University, Guilin, Guangxi 541004, P.R. China.
Oncol Lett ; 18(2): 1035-1042, 2019 Aug.
Article en En | MEDLINE | ID: mdl-31423163
ABSTRACT
Cadmium is a heavy metal that is toxic to humans and the reproductive system. The present study aimed to investigate the mechanisms of cadmium-induced reproductive toxicity in a male Institute of Cancer Research mouse model of cadmium poisoning. Changes in luteinizing hormone receptor (LHR), 17α-hydroxylase and endothelial nitric oxide (NO) synthase (eNOS) expression levels were examined. A total of 24 male mice (4-week-old) were randomly divided into four groups (normal control group and low, medium and high cadmium groups) and subjected to gavage treatment with normal saline or cadmium-containing saline solutions for 8 weeks prior to sacrifice. To assess testicular injury, serum androgen levels were determined by ELISA, testicular tissue pathological changes were evaluated using hematoxylin and eosin staining. In addition, LHR, 17α-hydroxylase and eNOS expressions levels were examined by western blotting, and apoptosis was examined with a terminal deoxynucleotidyl transferase dUTP nick end labeling assay. The results demonstrated that the severity of testes injury increased with cadmium concentration. In addition, LHR, 17α-hydroxylase and eNOS expression levels increased with low and medium concentrations of cadmium; however, they were decreased following treatment with high concentrations of cadmium. The results from the present study demonstrated that cadmium altered LHR, 17α-hydroxylase and eNOS expression levels in testicular stromal cells, which may impact testosterone synthesis. Furthermore, NO was suggested to be involved in cadmium-induced testicular injury by measurements of eNOS expression in testicular stromal cells.
Palabras clave