Acute effects on human sperm exposed in vitro to cadmium chloride and diisobutyl phthalate.
Reproduction
; 158(3): 281-290, 2019 09 01.
Article
en En
| MEDLINE
| ID: mdl-31437814
ABSTRACT
Epidemiological studies reported a negative relationship between concentrations of heavy metals and phthalates in seminal fluid and semen quality, likely compromising male fertility potential. The aim of this study was to investigate the in vitro effects of cadmium chloride (CdCl2), a common heavy metal, and diisobutyl phthalate (DIBP), a common phthalate ester, on human sperm functions necessary for fertilization. After in vitro incubation of spermatozoa with 10 µM CdCl2 or 100 and 200 µM DIBP for 24 h, a significant decrease of sperm progressive and hyperactivated motility was observed. The exposure to each of the two toxic agents also induced spontaneous sperm acrosome reaction and blunted the physiological response to progesterone. Both agents induced an increase of caspase activity suggesting triggering of an apoptotic pathway. Our results suggest that acute exposure of spermatozoa to these pollutants may impair sperm ability to reach and fertilize the oocyte.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Motilidad Espermática
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Espermatozoides
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Cloruro de Cadmio
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Reacción Acrosómica
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Dibutil Ftalato
Límite:
Humans
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Male
Idioma:
En
Año:
2019
Tipo del documento:
Article