Molecular Cloning, Lentiviral Transduction, and Expression of Recombinant ADAMTSL2 and ADAMTSL4.

Singh, Mukti; Cain, Stuart A; Baldock, Clair

Singh, Mukti; Cain, Stuart A; Baldock, Clair.

Singh M; Faculty of Biology, Medicine and Health, Wellcome Centre for Cell-Matrix Research, Division of Cell-Matrix Biology and Regenerative Medicine, School of Biological Sciences, University of Manchester, Manchester Academic Health Science Centre, Manchester, UK.
Cain SA; Faculty of Biology, Medicine and Health, Wellcome Centre for Cell-Matrix Research, Division of Cell-Matrix Biology and Regenerative Medicine, School of Biological Sciences, University of Manchester, Manchester Academic Health Science Centre, Manchester, UK.
Baldock C; Faculty of Biology, Medicine and Health, Wellcome Centre for Cell-Matrix Research, Division of Cell-Matrix Biology and Regenerative Medicine, School of Biological Sciences, University of Manchester, Manchester Academic Health Science Centre, Manchester, UK. clair.baldock@manchester.ac.uk.

Methods Mol Biol ; 2043: 137-155, 2020.

Article en En | MEDLINE | ID: mdl-31463909

Asunto(s)

Proteínas ADAMTS/genética; Clonación Molecular/métodos; Transducción Genética/métodos; Proteínas ADAMTS/metabolismo; Vectores Genéticos; Células HEK293; Humanos; Lentivirus/genética; Proteínas Recombinantes/metabolismo

Palabras clave

HEK293-EBNA; Lentiviral system; Lentiviral transduction; Mammalian; Molecular cloning; Recombinant expression; Stable cell lines

Texto completo

Imprimir

XML

PubMed Links

Search on Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Transducción Genética / Clonación Molecular / Proteínas ADAMTS Tipo de estudio: Guideline Límite: Humans Idioma: En Año: 2020 Tipo del documento: Article

Texto completo

Imprimir

XML

PubMed Links

Search on Google