Non-classical tissue monocytes and two functionally distinct populations of interstitial macrophages populate the mouse lung.
Nat Commun
; 10(1): 3964, 2019 09 03.
Article
en En
| MEDLINE
| ID: mdl-31481690
ABSTRACT
Resident tissue macrophages (RTM) can fulfill various tasks during development, homeostasis, inflammation and repair. In the lung, non-alveolar RTM, called interstitial macrophages (IM), importantly contribute to tissue homeostasis but remain little characterized. Here we show, using single-cell RNA-sequencing (scRNA-seq), two phenotypically distinct subpopulations of long-lived monocyte-derived IM, i.e. CD206+ and CD206-IM, as well as a discrete population of extravasating CD64+CD16.2+ monocytes. CD206+ IM are peribronchial self-maintaining RTM that constitutively produce high levels of chemokines and immunosuppressive cytokines. Conversely, CD206-IM preferentially populate the alveolar interstitium and exhibit features of antigen-presenting cells. In addition, our data support that CD64+CD16.2+ monocytes arise from intravascular Ly-6Clo patrolling monocytes that enter the tissue at steady-state to become putative precursors of CD206-IM. This study expands our knowledge about the complexity of lung IM and reveals an ontogenic pathway for one IM subset, an important step for elaborating future macrophage-targeted therapies.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Monocitos
/
Macrófagos Alveolares
/
Pulmón
Límite:
Animals
Idioma:
En
Año:
2019
Tipo del documento:
Article