Quantitative polymerase chain reaction technology in chimerism monitoring after hematopoietic stem cell transplantation: One center experience.
HLA
; 94 Suppl 2: 16-20, 2019 12.
Article
en En
| MEDLINE
| ID: mdl-31577854
ABSTRACT
Chimerism status evaluation is a routine test performed in post-hematopoietic stem cell transplantation (HSCT) period. The aim of the study was to evaluate a quantitative polymerase chain reaction (qPCR) method (GenDx, Utrecht, the Netherlands) applicability for this purpose. The study included 74 recipient/donor pairs tested for informative markers median of four and six informative markers was found for patients (related and unrelated donor, respectively). Higher sensitivity of qPCR method was confirmed by analysis of recipient post-HSCT samples (N = 800) among which microchimerism (0.1%-1% recipient DNA) was detected in 21.8% of cases. The ability to detect less than 1% of minor population, as opposed to the short tandem repeat (STR) method for which 1% is the limit, translated into earlier identification of a disease relapse for four patients in our study sample.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Quimera por Trasplante
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Trasplante de Células Madre Hematopoyéticas
/
Quimerismo
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Reacción en Cadena en Tiempo Real de la Polimerasa
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Monitoreo Fisiológico
Tipo de estudio:
Observational_studies
Límite:
Humans
Idioma:
En
Año:
2019
Tipo del documento:
Article