MiR-182-5p enhances in vitro neutrophil infiltration in Kawasaki disease.
Mol Genet Genomic Med
; 7(12): e990, 2019 12.
Article
en En
| MEDLINE
| ID: mdl-31605468
ABSTRACT
BACKGROUND:
Kawasaki disease (KD) patients could develop coronary artery lesion (CAL) which threatens children's life. A previous study identified KD biomarker miRNAs that could discriminate KD patients from febrile non-KD patients. We wonder whether these KD prediction biomarkers could be further applied to predict CAL formation in KD patients.METHODS:
To examine this hypothesis, we conducted a meta-analysis, miRNA mimic transfection, in vitro cell model and microarray assays.RESULTS:
We first showed that miR-182-5p and miR-183-5p kept higher levels in the KD patients with CAL than those without CAL (p < .05). Further machine learning alignment confirmed that CAL formation could be predicted, with an auROC value of 0.86. We further treated neutrophil cells with miR-182-5p mimic, followed by in vitro transendotherial migration assay. As a result, miR-182-5p overexpression significantly (p < .05) enhanced neutrophil cells to infiltrate the endothelial layer composed of human coronary artery endothelium cells. Further microarray assay and pathway enrichment analysis showed that the genes activated with miR-182-5p overexpression were significantly enriched in the leukocyte transendothelial migration pathway (kegg_pathway_194, p < .05).CONCLUSION:
Therefore, our study suggested that miR-182-5p enhanced in vitro leukocyte infiltration by activating the leukocyte transendothelial migration pathway in CAL formation in KD.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Regulación hacia Arriba
/
Estenosis Coronaria
/
MicroARNs
/
Síndrome Mucocutáneo Linfonodular
/
Neutrófilos
Tipo de estudio:
Etiology_studies
/
Prognostic_studies
Límite:
Child
/
Child, preschool
/
Female
/
Humans
/
Male
Idioma:
En
Año:
2019
Tipo del documento:
Article