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Pyomelanin Secretion in Madurella mycetomatis Interferes with Spectrophotometric Endpoint Reading Using the Sensititre YeastOne alamarBlue Assay but Not with Visual Endpoint Reading.
Nyuykonge, B; Croughs, P D; Fahal, A H; Verbon, A; van de Sande, W W J.
  • Nyuykonge B; Erasmus Medical Center, Department of Medical Microbiology and Infectious Diseases, Rotterdam, The Netherlands.
  • Croughs PD; Erasmus Medical Center, Department of Medical Microbiology and Infectious Diseases, Rotterdam, The Netherlands.
  • Fahal AH; Mycetoma Research Center, Khartoum, Sudan.
  • Verbon A; Erasmus Medical Center, Department of Medical Microbiology and Infectious Diseases, Rotterdam, The Netherlands.
  • van de Sande WWJ; Erasmus Medical Center, Department of Medical Microbiology and Infectious Diseases, Rotterdam, The Netherlands w.vandesande@erasmusmc.nl.
Antimicrob Agents Chemother ; 64(1)2019 12 20.
Article en En | MEDLINE | ID: mdl-31611353
ABSTRACT
The use of the Sensititre YeastOne YO10 alamarBlue assay for the in vitro susceptibility testing of Madurella mycetomatis was evaluated in M. mycetomatis isolates with and without pyomelanin secretion. Pyomelanin secretion did not influence visual endpoint reading; however, it caused a shift in peak absorbance from 570 nm to 620 nm when read spectrophotometrically. Therefore, when choosing the method for endpoint reading, the presence of pyomelanin should be considered.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Madurella / Micetoma / Melaninas Idioma: En Año: 2019 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Search on Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Madurella / Micetoma / Melaninas Idioma: En Año: 2019 Tipo del documento: Article