Integrin Antibody Decreases Deformability of Patient-Derived Pre-B Acute Lymphocytic Leukemia Cells as Measured by High-Frequency Acoustic Tweezers.
J Ultrasound Med
; 39(3): 589-595, 2020 Mar.
Article
en En
| MEDLINE
| ID: mdl-31633840
ABSTRACT
OBJECTIVES:
This article reports a study of cell mechanics in patient-derived (primary) B-cell acute lymphocytic leukemia (ALL) cells treated with antibodies against integrins. Leukemia cell adhesion to stromal cells mediates chemotherapeutic drug resistance, also known as cell adhesion-mediated chemotherapeutic drug resistance. We have previously shown that antibodies against integrin α4 and α6 adhesion molecules can de-adhere ALL cells from stromal cells or counter-receptors. Because drug-resistant cells are more deformable, as evaluated by single-beam acoustic tweezers, we hypothesized that changes in cell mechanics might contribute to the de-adhesive effect of integrin-targeting antibodies.METHODS:
In this study, the deformability of primary pre-B ALL cells was evaluated by single-beam acoustic tweezers after treatments with the de-adhering antibody Tysabri or P5G10 against integrin α4 and α6 adhesion molecules.RESULTS:
We demonstrated that primary ALL cells treated with P5G10 expressed decreased deformability compared with immunoglobulin G1 -treated control cells (P < .05). Tysabri did not show an effect on deformability (P > .05).CONCLUSIONS:
These results suggest that decreased deformability is associated with an integrin α6 blockade. Further assessments of the functional roles of deformability and integrin blockades in B-ALL cell drug resistance and deformability, respectively, are necessary.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Integrinas
/
Adhesión Celular
/
Leucemia-Linfoma Linfoblástico de Células Precursoras
/
Natalizumab
/
Factores Inmunológicos
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Año:
2020
Tipo del documento:
Article