Metal-Free Photoinduced Atom Transfer Radical Polymerization for Highly Sensitive Detection of Lung Cancer DNA.
Chemistry
; 26(7): 1633-1639, 2020 Feb 03.
Article
en En
| MEDLINE
| ID: mdl-31724757
ABSTRACT
Convenient and sensitive detection of biomolecules is of great significance to disease diagnosis. In this work, a metal-free photoinduced atom transfer radical polymerization (photoATRP) by a reductive quenching pathway as a novel strategy is applied to achieve lung cancer DNA detection. Thiolated PNA is exploited to specifically recognize target DNA, and the initiator of photoATRP is linked to the electrode surface via phosphate-Zr4+ -carboxylate. Under the excitation of blue light, the reductive quenching pathway is activated with eosin Y (EY) as photoredox catalyst and N,N,N',N'',N'-pentamethyldiethylenetriamine (PMDETA) as electron donor, and numerous polymeric chains are formed. Under optimal conditions, the linear range of this strategy is from 0.1â
pm to 10â
nm (R2 =0.989) with a limit of detection (LOD) of 1.4â
fm (14â
zmol in 10â
µL). The variety of possible light sources for photoATRP and simple operation endow this biosensor with great potential for practical applications.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Polímeros
/
ADN
/
Técnicas Biosensibles
/
Radicales Libres
/
Neoplasias Pulmonares
/
Metales
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Año:
2020
Tipo del documento:
Article