De novo formation of citrate-based fluorophores on N-termini of peptides and proteins in cells and tissues.
Chem Commun (Camb)
; 56(1): 74-77, 2020 Jan 04.
Article
en En
| MEDLINE
| ID: mdl-31790117
ABSTRACT
We developed a new method for the de novo formation of fluorophores based on citrate (DNFC) in biological samples. Use of an amide coupling reagent and microwave irradiation greatly facilitates the fluorophore formation on peptides and proteins with N-terminal cysteine or serine. Since N-terminal cysteine and serine can form thiazolopyridone- or oxazolopyridone-based fluorophores emitting blue and green fluorescence, respectively, by the DNFC staining, each organelle, cell and tissue exhibited a characteristic fluorescence distribution. The DNFC staining is able to provide a new potential protocol for future cell imaging, histology and diagnosis.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Péptidos
/
Sondas Moleculares
/
Proteínas
/
Colorantes Fluorescentes
Tipo de estudio:
Guideline
/
Prognostic_studies
Límite:
Animals
/
Humans
Idioma:
En
Año:
2020
Tipo del documento:
Article