Phylogenetic classification of a group of self-replicating RNAs that are common in co-infections with poleroviruses.
Virus Res
; 276: 197831, 2020 01 15.
Article
en En
| MEDLINE
| ID: mdl-31790776
ABSTRACT
Tombusvirus-like associated RNAs (tlaRNAs) are positive-sense single-stranded RNAs found in plants co-infected with viruses of the genus Polerovirus. TlaRNAs depend upon capsid proteins supplied in trans by the co-infecting polerovirus vector for transmission and intra-host systemic movement. Here, the full-length genomes of five tlaRNAs were determined using a combination of RT-PCR and next-generation sequencing, and evidence is provided for an additional tlaRNA associated with potato leafroll virus. Phylogenetic analyses based on conserved domains of the RdRp placed tlaRNAs as a monophyletic clade clustering with members of the family Tombusviridae and comprising three different subclades. Full-length clones of tlaRNAs from two of three subclades were confirmed to replicate autonomously, and each produces a subgenomic RNA during infection.
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Banco de datos:
MEDLINE
Asunto principal:
Filogenia
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ARN Viral
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Genoma Viral
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Luteoviridae
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Coinfección
Idioma:
En
Año:
2020
Tipo del documento:
Article