Molecular Orientation Determination in Nanodiscs at the Single-Molecule Level.
Anal Chem
; 92(2): 2229-2236, 2020 01 21.
Article
en En
| MEDLINE
| ID: mdl-31851490
ABSTRACT
The function of membrane-bound proteins often depends on their interactions with the lipid bilayer. Bulk absorption-based linear dichroism has been historically used to investigate molecular orientations in the phospholipid bilayer but cannot resolve the actual distribution of molecules embedded in the membrane and is often limited by a poor signal-to-noise ratio. Here, we present single-molecule orientation determination by fluorescence-detected linear dichroism visualization in Nanodisc grids or SOLVING, to determine the molecular orientation of molecules assembled into nanoscale lipid bilayers. We provide a proof-of-concept by using SOLVING to quantitate the orientation distribution of two commonly used fluorescent dyes, DiO and BODIPY, in 10 nm Nanodiscs. Besides confirming the mean orientation determined by bulk absorption measurement, SOLVING provides the actual distribution of orientations and promises to provide key molecular insights into the topology and interactions of multiprotein complexes, such as those observed in intracellular signal transduction.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Nanoestructuras
/
Membrana Dobles de Lípidos
Idioma:
En
Año:
2020
Tipo del documento:
Article