A polar stationary phase obtained by surface-initiated polymerization of hyperbranched polyglycerol onto silica.

Geng, Huiliang; Jing, Jie; Zhang, Feifang; Zhang, Feng; Yang, Bingcheng.

Geng H; Engineering Research Center of Pharmaceutical Process Chemistry, East China University of Science and Technology, Shanghai, 200237, China.
Jing J; Engineering Research Center of Pharmaceutical Process Chemistry, East China University of Science and Technology, Shanghai, 200237, China.
Zhang F; Engineering Research Center of Pharmaceutical Process Chemistry, East China University of Science and Technology, Shanghai, 200237, China. Electronic address: zhangff@ecust.edu.cn.
Zhang F; Institute of Food Safety, Chinese Academy of Inspection & Quarantine, Beijing, 100176, China.
Yang B; Engineering Research Center of Pharmaceutical Process Chemistry, East China University of Science and Technology, Shanghai, 200237, China. Electronic address: bcyang@ecust.edu.cn.

Talanta ; 209: 120525, 2020 Mar 01.

Article en En | MEDLINE | ID: mdl-31892073

ABSTRACT

A novel polar stationary phase for hydrophilic interaction chromatography (HILIC) is prepared by surface-initiated polymerization of hyperbranched polyglycerol onto silica. Such polymerization offers an easy way to tune the cabon load. The stationary phase showed obvious hydrogen bonding interaction and typical HILIC character, exhibiting nealy-universal separation ability towards polar analytes. Good peak shape and high separation efficiency was observed for model polar analytes, including nucleosides and nucleic bases, aromatic acids, organic bases, vitamins, saccharides, amino acids, e.g. 35,000-58,000 plate counts per meter for five water-soluble vitamins.

Palabras clave

Hydrophilic interaction liquid chromatography; Hyperbranched polyglycerol; Stationary phase; Surface-initiated polymerization

Texto completo: 1 Banco de datos: MEDLINE Idioma: En Año: 2020 Tipo del documento: Article