A New Way to Discover IRESs in Pathology or Stress Conditions? Harnessing Latest High-Throughput Technologies.
Bioessays
; 42(3): e1900180, 2020 03.
Article
en En
| MEDLINE
| ID: mdl-31909834
ABSTRACT
The cellular internal ribosomal entry site (IRES) is one of the most important elements to mediate cap-independent translational initiation, especially under conditions of stress and pathology. However, a high-throughput method to discover IRESs in these conditions is still lacking. Here, a possible way IRES long-read sequencing based on the latest high-throughput technologies is proposed to solve this problem. Based on this design, diversity and integrity of the transcriptome from original samples can be kept. The micro-environment that stimulates or inhibits IRES activity can also be mimicked. By using long read-length sequencing technology, additional experiments that are essential for ruling out the cryptic promoters or splicing events in routine IRES identification processes can be circumvented. It is hoped that this proposed methodology may be adopted for IRES element discovery, hence uncovering the full extent of the role of IRESs in disease, development, and stress. Also see the video abstract here https//youtu.be/JuWBbMzWXS8.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Ribosomas
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Secuenciación de Nucleótidos de Alto Rendimiento
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Sitios Internos de Entrada al Ribosoma
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Neoplasias
Tipo de estudio:
Prognostic_studies
Límite:
Animals
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Humans
Idioma:
En
Año:
2020
Tipo del documento:
Article