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A novel fluorescent chemosensor based on coumarin and quinolinyl-benzothiazole for sequential recognition of Cu2+ and PPi and its applicability in live cell imaging.
Li, Shengling; Cao, Duanlin; Meng, Xianjiao; Hu, Zhiyong; Li, Zhichun; Yuan, Changchun; Zhou, Tao; Han, Xinghua; Ma, Wenbing.
  • Li S; School of Chemical Engineering and Technology, North University of China, Taiyuan 030051, PR China.
  • Cao D; School of Chemical Engineering and Technology, North University of China, Taiyuan 030051, PR China.
  • Meng X; College of Arts and Sciences, Shanxi Agricultural University, Taigu, Shanxi 030801, PR China.
  • Hu Z; School of Chemical Engineering and Technology, North University of China, Taiyuan 030051, PR China; National Demonstration Center for Experimental Comprehensive Chemical Engineering Education, North University of China, Taiyuan 030051, PR China.
  • Li Z; School of Chemical Engineering and Technology, North University of China, Taiyuan 030051, PR China.
  • Yuan C; School of Chemical Engineering and Technology, North University of China, Taiyuan 030051, PR China.
  • Zhou T; School of Chemical Engineering and Technology, North University of China, Taiyuan 030051, PR China.
  • Han X; School of Chemical Engineering and Technology, North University of China, Taiyuan 030051, PR China; National Demonstration Center for Experimental Comprehensive Chemical Engineering Education, North University of China, Taiyuan 030051, PR China.
  • Ma W; School of Chemical Engineering and Technology, North University of China, Taiyuan 030051, PR China; National Demonstration Center for Experimental Comprehensive Chemical Engineering Education, North University of China, Taiyuan 030051, PR China. Electronic address: mawenbing@nuc.edu.cn.
Spectrochim Acta A Mol Biomol Spectrosc ; 230: 118022, 2020 Apr 05.
Article en En | MEDLINE | ID: mdl-31927510
ABSTRACT
In this study, a highly selective fluorescent sensor (E)-2-((2-(benzo[d]thiazol-2-yl)quinolin-8-yl)oxy)-N'-((7-(diethylamino)-2-oxo-2H-chromen-3-yl)methylene)acetohydrazide (TQC) was synthesized from 2-methylquinolin-8-ol and 4-(diethylamino)-2-hydroxybenzaldehyde and its structure was characterized by 1H NMR, 13C NMR, ESI-HR-MS and density functional theory (DFT) calculation. Sensor TQC showed an obvious "on-off-on" fluorescence response to Cu2+ and PPi in a DMSO/HEPES (32 v/v, pH = 7.4) buffer system. The detection limits of sensor TQC were 0.06 µM to Cu2+ and 0.01 µM to PPi. In addition, sensor TQC showed a 11 binding stoichiometry to Cu2+ and TQC-Cu2+ complex showed a 21 binding stoichiometry to PPi. The optimum pH range of sensor TQC and TQC-Cu2+ was 3-8. Further studies demonstrated that sensor TQC could be made into test paper strips for the qualitative of Cu2+ and PPi and showed sequentially "on-off-on" fluorescent bio-imaging of Cu2+ and PPi in HeLa cells.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Difosfatos / Cobre / Cumarinas / Benzotiazoles / Colorantes Fluorescentes Tipo de estudio: Qualitative_research Límite: Humans Idioma: En Año: 2020 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Search on Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Difosfatos / Cobre / Cumarinas / Benzotiazoles / Colorantes Fluorescentes Tipo de estudio: Qualitative_research Límite: Humans Idioma: En Año: 2020 Tipo del documento: Article