A novel fluorescent chemosensor based on coumarin and quinolinyl-benzothiazole for sequential recognition of Cu2+ and PPi and its applicability in live cell imaging.
Spectrochim Acta A Mol Biomol Spectrosc
; 230: 118022, 2020 Apr 05.
Article
en En
| MEDLINE
| ID: mdl-31927510
ABSTRACT
In this study, a highly selective fluorescent sensor (E)-2-((2-(benzo[d]thiazol-2-yl)quinolin-8-yl)oxy)-N'-((7-(diethylamino)-2-oxo-2H-chromen-3-yl)methylene)acetohydrazide (TQC) was synthesized from 2-methylquinolin-8-ol and 4-(diethylamino)-2-hydroxybenzaldehyde and its structure was characterized by 1H NMR, 13C NMR, ESI-HR-MS and density functional theory (DFT) calculation. Sensor TQC showed an obvious "on-off-on" fluorescence response to Cu2+ and PPi in a DMSO/HEPES (32 v/v, pH = 7.4) buffer system. The detection limits of sensor TQC were 0.06 µM to Cu2+ and 0.01 µM to PPi. In addition, sensor TQC showed a 11 binding stoichiometry to Cu2+ and TQC-Cu2+ complex showed a 21 binding stoichiometry to PPi. The optimum pH range of sensor TQC and TQC-Cu2+ was 3-8. Further studies demonstrated that sensor TQC could be made into test paper strips for the qualitative of Cu2+ and PPi and showed sequentially "on-off-on" fluorescent bio-imaging of Cu2+ and PPi in HeLa cells.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Difosfatos
/
Cobre
/
Cumarinas
/
Benzotiazoles
/
Colorantes Fluorescentes
Tipo de estudio:
Qualitative_research
Límite:
Humans
Idioma:
En
Año:
2020
Tipo del documento:
Article