Technology used to build and transfer mammalian chromosomes.
Exp Cell Res
; 388(2): 111851, 2020 03 15.
Article
en En
| MEDLINE
| ID: mdl-31952951
ABSTRACT
In the near twenty-year existence of the human and mammalian artificial chromosome field, the technologies for artificial chromosome construction and installation into desired cell types or organisms have evolved with the rest of modern molecular and synthetic biology. Medical, industrial, pharmaceutical, agricultural, and basic research scientists seek the as yet unrealized promise of human and mammalian artificial chromosomes. Existing technologies for both top-down and bottom-up approaches to construct these artificial chromosomes for use in higher eukaryotes are very different but aspire to achieve similar results. New capacity for production of chromosome sized synthetic DNA will likely shift the field towards more bottom-up approaches, but not completely. Similarly, new approaches to install human and mammalian artificial chromosomes in target cells will compete with the microcell mediated cell transfer methods that currently dominate the field.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Terapia Genética
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Técnicas de Transferencia de Gen
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Cromosomas Artificiales de los Mamíferos
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Cromosomas de los Mamíferos
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Vectores Genéticos
Límite:
Animals
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Humans
Idioma:
En
Año:
2020
Tipo del documento:
Article