Stable Isotopes for Tracing Mammalian-Cell Metabolism In Vivo.
Trends Biochem Sci
; 45(3): 185-201, 2020 03.
Article
en En
| MEDLINE
| ID: mdl-31955965
ABSTRACT
Metabolism is at the cornerstone of all cellular functions and mounting evidence of its deregulation in different diseases emphasizes the importance of a comprehensive understanding of metabolic regulation at the whole-organism level. Stable-isotope measurements are a powerful tool for probing cellular metabolism and, as a result, are increasingly used to study metabolism in in vivo settings. The additional complexity of in vivo metabolic measurements requires paying special attention to experimental design and data interpretation. Here, we review recent work where in vivo stable-isotope measurements have been used to address relevant biological questions within an in vivo context, summarize different experimental and data interpretation approaches and their limitations, and discuss future opportunities in the field.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Células
/
Marcaje Isotópico
Límite:
Animals
/
Humans
Idioma:
En
Año:
2020
Tipo del documento:
Article