Amorphous silica nanoparticle-induced pulmonary inflammatory response depends on particle size and is sex-specific in rats.
Toxicol Appl Pharmacol
; 390: 114890, 2020 03 01.
Article
en En
| MEDLINE
| ID: mdl-31972177
ABSTRACT
Due to mass production and extensive use, the potential adverse health effects of amorphous silica nanoparticles (ASiNPs) have received a significant attention from the public and researchers. However, the relationship between physicochemical properties of ASiNPs and their health effects is still unclear. In this study, we manufactured two types of ASiNPs of different diameters (20 and 50â¯nm) and compared the toxic response induced in rats after intratracheal instillation (75, 150 or 300⯵g/rat). There were no dose-related differences in mortality, body weight gain or organ weight between the groups. However both types of ASiNPs significantly decreased the proportion of neutrophils in male rats, whereas the levels of hemoglobin and hematocrit were markedly reduced only in female rats instilled with 20â¯nm-ASiNPs. ASiNPs-induced lung tissue damage seemed to be more evident in the 20â¯nm ASiNP-treated group and in female rats than male rats. Similarly, expression of caveolin-1 and matrix metalloproteinase-9 seemed to be most notably enhanced in female rats treated with 20â¯nm-ASiNPs. The total number of bronchial alveolar lavage cells significantly increased in rats instilled with 20â¯nm-ASiNPs, accompanying a decrease in the proportion of macrophages and an increase in polymorphonuclear leukocytes. Moreover, secretion of inflammatory mediators clearly increased in human bronchial epithelial cells treated with 20â¯nm-ASiNPs, but not in those treated with 50â¯nm-ASiNPs. These results suggest that pulmonary effects of ASiNPs depend on particle size. Sex-dependent differences should also be carefully considered in understanding nanomaterial-induced adverse health effects.
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Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Tamaño de la Partícula
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Dióxido de Silicio
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Nanopartículas
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Inflamación
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Enfermedades Pulmonares
Límite:
Animals
Idioma:
En
Año:
2020
Tipo del documento:
Article