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Dissecting transcriptomic signatures of neuronal differentiation and maturation using iPSCs.
Burke, Emily E; Chenoweth, Joshua G; Shin, Joo Heon; Collado-Torres, Leonardo; Kim, Suel-Kee; Micali, Nicola; Wang, Yanhong; Colantuoni, Carlo; Straub, Richard E; Hoeppner, Daniel J; Chen, Huei-Ying; Sellers, Alana; Shibbani, Kamel; Hamersky, Gregory R; Diaz Bustamante, Marcelo; Phan, BaDoi N; Ulrich, William S; Valencia, Cristian; Jaishankar, Amritha; Price, Amanda J; Rajpurohit, Anandita; Semick, Stephen A; Bürli, Roland W; Barrow, James C; Hiler, Daniel J; Page, Stephanie C; Martinowich, Keri; Hyde, Thomas M; Kleinman, Joel E; Berman, Karen F; Apud, Jose A; Cross, Alan J; Brandon, Nicholas J; Weinberger, Daniel R; Maher, Brady J; McKay, Ronald D G; Jaffe, Andrew E.
  • Burke EE; Lieber Institute for Brain Development, Baltimore, MD, USA.
  • Chenoweth JG; Lieber Institute for Brain Development, Baltimore, MD, USA.
  • Shin JH; Lieber Institute for Brain Development, Baltimore, MD, USA.
  • Collado-Torres L; Lieber Institute for Brain Development, Baltimore, MD, USA.
  • Kim SK; Lieber Institute for Brain Development, Baltimore, MD, USA.
  • Micali N; Lieber Institute for Brain Development, Baltimore, MD, USA.
  • Wang Y; Lieber Institute for Brain Development, Baltimore, MD, USA.
  • Colantuoni C; Lieber Institute for Brain Development, Baltimore, MD, USA.
  • Straub RE; Lieber Institute for Brain Development, Baltimore, MD, USA.
  • Hoeppner DJ; Lieber Institute for Brain Development, Baltimore, MD, USA.
  • Chen HY; Lieber Institute for Brain Development, Baltimore, MD, USA.
  • Sellers A; Lieber Institute for Brain Development, Baltimore, MD, USA.
  • Shibbani K; Lieber Institute for Brain Development, Baltimore, MD, USA.
  • Hamersky GR; Lieber Institute for Brain Development, Baltimore, MD, USA.
  • Diaz Bustamante M; Lieber Institute for Brain Development, Baltimore, MD, USA.
  • Phan BN; Lieber Institute for Brain Development, Baltimore, MD, USA.
  • Ulrich WS; Lieber Institute for Brain Development, Baltimore, MD, USA.
  • Valencia C; Lieber Institute for Brain Development, Baltimore, MD, USA.
  • Jaishankar A; Lieber Institute for Brain Development, Baltimore, MD, USA.
  • Price AJ; Lieber Institute for Brain Development, Baltimore, MD, USA.
  • Rajpurohit A; McKusick Nathans Institute of Genetic Medicine, Johns Hopkins School of Medicine, Baltimore, MD, USA.
  • Semick SA; Lieber Institute for Brain Development, Baltimore, MD, USA.
  • Bürli RW; Lieber Institute for Brain Development, Baltimore, MD, USA.
  • Barrow JC; Neuroscience, IMED Biotech Unit, AstraZeneca, Cambridge, UK.
  • Hiler DJ; Lieber Institute for Brain Development, Baltimore, MD, USA.
  • Page SC; Lieber Institute for Brain Development, Baltimore, MD, USA.
  • Martinowich K; Lieber Institute for Brain Development, Baltimore, MD, USA.
  • Hyde TM; Lieber Institute for Brain Development, Baltimore, MD, USA.
  • Kleinman JE; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, MD, USA.
  • Berman KF; Department of Neuroscience, Johns Hopkins School of Medicine, Baltimore, MD, USA.
  • Apud JA; Lieber Institute for Brain Development, Baltimore, MD, USA.
  • Cross AJ; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, MD, USA.
  • Brandon NJ; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, MD, USA.
  • Weinberger DR; Lieber Institute for Brain Development, Baltimore, MD, USA.
  • Maher BJ; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, MD, USA.
  • McKay RDG; Clinical and Translational Neuroscience Branch, NIMH Intramural Research Program, Bethesda, MD, USA.
  • Jaffe AE; Clinical and Translational Neuroscience Branch, NIMH Intramural Research Program, Bethesda, MD, USA.
Nat Commun ; 11(1): 462, 2020 01 23.
Article en En | MEDLINE | ID: mdl-31974374
ABSTRACT
Human induced pluripotent stem cells (hiPSCs) are a powerful model of neural differentiation and maturation. We present a hiPSC transcriptomics resource on corticogenesis from 5 iPSC donor and 13 subclonal lines across 9 time points over 5 broad conditions self-renewal, early neuronal differentiation, neural precursor cells (NPCs), assembled rosettes, and differentiated neuronal cells. We identify widespread changes in the expression of both individual features and global patterns of transcription. We next demonstrate that co-culturing human NPCs with rodent astrocytes results in mutually synergistic maturation, and that cell type-specific expression data can be extracted using only sequencing read alignments without cell sorting. We lastly adapt a previously generated RNA deconvolution approach to single-cell expression data to estimate the relative neuronal maturity of iPSC-derived neuronal cultures and human brain tissue. Using many public datasets, we demonstrate neuronal cultures are maturationally heterogeneous but contain subsets of neurons more mature than previously observed.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Diferenciación Celular / Células Madre Pluripotentes Inducidas / Células-Madre Neurales / Transcriptoma Límite: Animals / Humans Idioma: En Año: 2020 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Search on Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Diferenciación Celular / Células Madre Pluripotentes Inducidas / Células-Madre Neurales / Transcriptoma Límite: Animals / Humans Idioma: En Año: 2020 Tipo del documento: Article