Key significance of DNA-target size in lateral flow assay coupled with recombinase polymerase amplification.

Safenkova, Irina V; Ivanov, Alexandr V; Slutskaya, Elvira S; Samokhvalov, Alexey V; Zherdev, Anatoly V; Dzantiev, Boris B.

Safenkova IV; A.N. Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of Sciences, Leninsky prospect 33, 119071, Moscow, Russia.
Ivanov AV; A.N. Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of Sciences, Leninsky prospect 33, 119071, Moscow, Russia.
Slutskaya ES; A.N. Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of Sciences, Leninsky prospect 33, 119071, Moscow, Russia.
Samokhvalov AV; A.N. Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of Sciences, Leninsky prospect 33, 119071, Moscow, Russia.
Zherdev AV; A.N. Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of Sciences, Leninsky prospect 33, 119071, Moscow, Russia.
Dzantiev BB; A.N. Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of Sciences, Leninsky prospect 33, 119071, Moscow, Russia. Electronic address: dzantiev@inbi.ras.ru.

Anal Chim Acta ; 1102: 109-118, 2020 Mar 15.

Article en En | MEDLINE | ID: mdl-32043989

Asunto(s)

ADN/análisis; Anticuerpos Inmovilizados/inmunología; Biotina/química; ADN/química; Fluoresceínas/química; Colorantes Fluorescentes/química; Oro/química; Inmunoensayo/métodos; Límite de Detección; Nanopartículas del Metal/química; Técnicas de Amplificación de Ácido Nucleico/métodos; Estreptavidina/química

Palabras clave

DNA target; Isothermal amplification; Lateral flow assay; Point-of-care assays; Recombinase polymerase amplification