A preclinical large-animal model for the assessment of critical-size load-bearing bone defect reconstruction.
Nat Protoc
; 15(3): 877-924, 2020 03.
Article
en En
| MEDLINE
| ID: mdl-32060491
ABSTRACT
Critical-size bone defects, which require large-volume tissue reconstruction, remain a clinical challenge. Bone engineering has the potential to provide new treatment concepts, yet clinical translation requires anatomically and physiologically relevant preclinical models. The ovine critical-size long-bone defect model has been validated in numerous studies as a preclinical tool for evaluating both conventional and novel bone-engineering concepts. With sufficient training and experience in large-animal studies, it is a technically feasible procedure with a high level of reproducibility when appropriate preoperative and postoperative management protocols are followed. The model can be established by following a procedure that includes the following stages (i) preoperative planning and preparation, (ii) the surgical approach, (iii) postoperative management, and (iv) postmortem analysis. Using this model, full results for peer-reviewed publication can be attained within 2 years. In this protocol, we comprehensively describe how to establish proficiency using the preclinical model for the evaluation of a range of bone defect reconstruction options.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Huesos
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Procedimientos Ortopédicos
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Ingeniería de Tejidos
/
Fracturas Óseas
Tipo de estudio:
Guideline
Límite:
Animals
Idioma:
En
Año:
2020
Tipo del documento:
Article