Irisin promotes cementoblast differentiation via p38 MAPK pathway.
Oral Dis
; 26(5): 974-982, 2020 Jul.
Article
en En
| MEDLINE
| ID: mdl-32068933
ABSTRACT
OBJECTIVE:
Irisin is a newly identified exercise-induced myokine which can affect glucose metabolism and cortical bone mass and strength. However, the influence of irisin on cementoblasts remains largely unknown. MATERIAL ANDMETHODS:
An immortalized mouse cementoblast cell line OCCM-30 was used in this study. Cementoblast differentiation markers and PGC-1α in cells cultured with mineral induction medium were evaluated by qRT-PCR. Cementoblast mineralization was evaluated by alizarin red staining. Differentiation markers and the activity of p38 MAPK pathway under irisin stimulation were assessed by qRT-PCR or Western blot analysis. p38 MAPK pathway inhibitor SB203580 or p38 siRNA was used to further identify the regulatory mechanism. Cell proliferation treated with irisin was examined by CCK-8 method.RESULTS:
The expression of Runx2, osterix, ALP, and PGC-1α was up-regulated consistently under mineral induction. The formation of mineralized nodules was increased by irisin. Runx2, osterix, ALP, and osteocalcin were obviously up-regulated under irisin stimulation as well as the activity of p38 MAPK pathway. When pretreated with SB203580 or p38 siRNA before irisin stimulation, the irisin-induced differentiation was distinctly suppressed. OCCM-30 cell proliferation was enhanced when treated with high-dose irisin for long time.CONCLUSION:
Irisin can promote the differentiation of cementoblasts via p38 MAPK pathway.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Diferenciación Celular
/
Fibronectinas
/
Cemento Dental
/
Proteínas Quinasas p38 Activadas por Mitógenos
Límite:
Animals
Idioma:
En
Año:
2020
Tipo del documento:
Article