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Generation of a male common marmoset embryonic stem cell line DSY127-BV8VT1 carrying double reporters specific for the germ cell linage using the CRISPR-Cas9 and PiggyBac transposase systems.
Yoshimatsu, Sho; Sato, Tsukika; Yamamoto, Masafumi; Sasaki, Erika; Nakajima, Mayutaka; Nakamura, Mari; Shiozawa, Seiji; Noce, Toshiaki; Okano, Hideyuki.
  • Yoshimatsu S; Department of Physiology, School of Medicine, Keio University, Tokyo, Japan; Laboratory for Proteolytic Neuroscience, RIKEN Center for Brain Science, Saitama, Japan. Electronic address: yoshima@a7.keio.jp.
  • Sato T; Department of Physiology, School of Medicine, Keio University, Tokyo, Japan.
  • Yamamoto M; ICLAS Monitoring Center, Central Institute for Experimental Animals, Kanagawa, Japan.
  • Sasaki E; Department of Marmoset Biology and Medicine, Central Institute for Experimental Animals, Kanagawa, Japan.
  • Nakajima M; Department of Physiology, School of Medicine, Keio University, Tokyo, Japan.
  • Nakamura M; Department of Physiology, School of Medicine, Keio University, Tokyo, Japan.
  • Shiozawa S; Department of Physiology, School of Medicine, Keio University, Tokyo, Japan.
  • Noce T; Laboratory for Marmoset Neural Architecture, RIKEN Center for Brain Science, Saitama, Japan.
  • Okano H; Department of Physiology, School of Medicine, Keio University, Tokyo, Japan; Laboratory for Marmoset Neural Architecture, RIKEN Center for Brain Science, Saitama, Japan. Electronic address: hidokano@a2.keio.jp.
Stem Cell Res ; 44: 101740, 2020 04.
Article en En | MEDLINE | ID: mdl-32151954
ABSTRACT
BLIMP1 (PRDM1) and VASA (DDX4) play pivotal roles in the development of the germ cell linage. Importantly, these genes are specifically expressed in germ cells; BLIMP1 in primordial germ cells (PGCs) to early-stage gonocytes, and VASA in migration-stage PGCs to mature gametes. The high reproductive efficiency of common marmosets (marmosets; Callithrix jacchus) makes them advantageous for use in germ cell research. We herein report the generation of a male marmoset embryonic stem cell (ESC) line harboring BLIMP1 and DDX4 double reporters. This ESC line will be a useful tool for investigating male gametogenesis in non-human primates.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Callithrix / Línea Celular / Transposasas / Células Madre Embrionarias Límite: Animals Idioma: En Año: 2020 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Search on Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Callithrix / Línea Celular / Transposasas / Células Madre Embrionarias Límite: Animals Idioma: En Año: 2020 Tipo del documento: Article