Polycistronic transcripts in trypanosomes and their accumulation during heat shock: evidence for a precursor role in mRNA synthesis.
Mol Cell Biol
; 8(9): 3837-46, 1988 Sep.
Article
en En
| MEDLINE
| ID: mdl-3221866
ABSTRACT
Maturation of mRNA precursors in trypanosomes involves an apparent trans splicing event in which a 39-nucleotide miniexon sequence, common to all trypanosome mRNAs, is joined to the 5' end of a protein-coding exon. We demonstrate that the processing machinery responsible for the maturation of tubulin mRNA precursors in Trypanosoma brucei can be disrupted by heat shock. This results in an accumulation of polycistronic RNA species and a decrease in the abundance of branched splicing intermediates. At normal temperatures, tubulin polycistronic transcripts were also detected and were shown in pulse-chase experiments to be abundantly synthesized and very rapidly turned over. These results, combined with results of the heat shock experiments, suggest that these polycistronic transcripts are the precursors of the (monocistronic) tubulin mRNAs of trypanosomes.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Transcripción Genética
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Trypanosoma brucei brucei
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Tubulina (Proteína)
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ARN Mensajero
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Precursores del ARN
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Genes
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Proteínas de Choque Térmico
Límite:
Animals
Idioma:
En
Año:
1988
Tipo del documento:
Article