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Identification and complete genomic sequence of a novel sadwavirus discovered in pineapple (Ananas comosus).
Larrea-Sarmiento, Adriana; Olmedo-Velarde, Alejandro; Green, James C; Al Rwahnih, Maher; Wang, Xupeng; Li, Yun-He; Wu, Weihuai; Zhang, Jingxin; Matsumoto, Tracie K; Suzuki, Jon Y; Wall, Marisa M; Borth, Wayne; Melzer, Michael J; Hu, John S.
  • Larrea-Sarmiento A; Department of Plant and Environmental Protection Sciences, University of Hawaii, Honolulu, HI, USA.
  • Olmedo-Velarde A; Department of Plant and Environmental Protection Sciences, University of Hawaii, Honolulu, HI, USA.
  • Green JC; Department of Plant and Environmental Protection Sciences, University of Hawaii, Honolulu, HI, USA.
  • Al Rwahnih M; Department of Plant Pathology, University of California, Davis, CA, USA.
  • Wang X; Department of Plant and Environmental Protection Sciences, University of Hawaii, Honolulu, HI, USA.
  • Li YH; South Subtropical Crop Research Institute, Chinese Academy of Tropical Agricultural Science, Zhanjiang, China.
  • Wu W; Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.
  • Zhang J; Key Laboratory of New Technique for Plant Protection in Guangdong, Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
  • Matsumoto TK; United States Department of Agriculture, Agricultural Research Service, Pacific Basin Agricultural Research Center, Hilo, HI, USA.
  • Suzuki JY; United States Department of Agriculture, Agricultural Research Service, Pacific Basin Agricultural Research Center, Hilo, HI, USA.
  • Wall MM; United States Department of Agriculture, Agricultural Research Service, Pacific Basin Agricultural Research Center, Hilo, HI, USA.
  • Borth W; Department of Plant and Environmental Protection Sciences, University of Hawaii, Honolulu, HI, USA.
  • Melzer MJ; Department of Plant and Environmental Protection Sciences, University of Hawaii, Honolulu, HI, USA.
  • Hu JS; Department of Plant and Environmental Protection Sciences, University of Hawaii, Honolulu, HI, USA. johnhu@hawaii.edu.
Arch Virol ; 165(5): 1245-1248, 2020 May.
Article en En | MEDLINE | ID: mdl-32227308
ABSTRACT
The complete genomic sequence of a putative novel member of the family Secoviridae was determined by high-throughput sequencing of a pineapple accession obtained from the National Plant Germplasm Repository in Hilo, Hawaii. The predicted genome of the putative virus was composed of two RNA molecules of 6,128 and 4,161 nucleotides in length, excluding the poly-A tails. Each genome segment contained one large open reading frame (ORF) that shares homology and phylogenetic identity with members of the family Secoviridae. The presence of this new virus in pineapple was confirmed using RT-PCR and Sanger sequencing from six samples collected in Oahu, Hawaii. The name "pineapple secovirus A" (PSVA) is proposed for this putative new sadwavirus.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Genoma Viral / Análisis de Secuencia de ADN / Secoviridae / Ananas Tipo de estudio: Diagnostic_studies País como asunto: America do norte Idioma: En Año: 2020 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Search on Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Genoma Viral / Análisis de Secuencia de ADN / Secoviridae / Ananas Tipo de estudio: Diagnostic_studies País como asunto: America do norte Idioma: En Año: 2020 Tipo del documento: Article