Division of Labor between PCNA Loaders in DNA Replication and Sister Chromatid Cohesion Establishment.
Mol Cell
; 78(4): 725-738.e4, 2020 05 21.
Article
en En
| MEDLINE
| ID: mdl-32277910
ABSTRACT
Concomitant with DNA replication, the chromosomal cohesin complex establishes cohesion between newly replicated sister chromatids. Several replication-fork-associated "cohesion establishment factors," including the multifunctional Ctf18-RFC complex, aid this process in as yet unknown ways. Here, we show that Ctf18-RFC's role in sister chromatid cohesion correlates with PCNA loading but is separable from its role in the replication checkpoint. Ctf18-RFC loads PCNA with a slight preference for the leading strand, which is dispensable for DNA replication. Conversely, the canonical Rfc1-RFC complex preferentially loads PCNA onto the lagging strand, which is crucial for DNA replication but dispensable for sister chromatid cohesion. The downstream effector of Ctf18-RFC is cohesin acetylation, which we place toward a late step during replication maturation. Our results suggest that Ctf18-RFC enriches and balances PCNA levels at the replication fork, beyond the needs of DNA replication, to promote establishment of sister chromatid cohesion and possibly other post-replicative processes.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Saccharomyces cerevisiae
/
Proteínas Cromosómicas no Histona
/
Cromosomas Fúngicos
/
Cromátides
/
Antígeno Nuclear de Célula en Proliferación
/
Proteínas de Ciclo Celular
/
Proteínas de Saccharomyces cerevisiae
/
Replicación del ADN
Idioma:
En
Año:
2020
Tipo del documento:
Article